PB-MAX™ Karyotyping Medium 

Gibco® PB-MAX™ Karyotyping Medium is a fully-supplemented medium developed for the short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures.

Gibco® PB-MAX™ Karyotyping Medium features:
• Ready-to-use format
• Quality and performance testing
• Classic formulation
Ready-to-use format
Gibco® PB-MAX™ Karyotyping Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 14 days. 
Quality and performance testing
Every lot of Gibco® PB-MAX™ Karyotyping Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Peripheral blood lymphocytes from a normal adult donor are cultured for 72 hours in Gibco® PB-MAX™ Karyotyping Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, and mycoplasma.
Classic formulation
Gibco® PB-MAX™ Karyotyping Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), L-glutamine, and phytohemagglutinin (PHA). Gibco® PB-MAX™ Karyotyping Medium is based on formulations referenced in the Association for Cytogenetic Technologists Laboratory Manual for the culture of peripheral blood lymphocytes for cytogenetic analysis.  

Product list:

NO Cat.No           Product
1 Gibco(10270-106)                     Foetal Bovine Serum
Gibco(11269-016)                AmnioMAX™-II Complete Medium 
Gibco(12557-013)                PB-MAX™ Karyotyping Medium 
Gibco(15212-012)               KaryoMAX® Colcemid™ Solution in PBS 
 5   Gibco(15210-040)               KaryoMAX® Colcemid™ Solution in HBSS 
 6  Gibco(10576-015)                KaryomAX(PHA) 
 7  Gibco(25200-056)                Trypsin-EDTA (0.25%), phenol red 
  8  Gibco(25300-054)                Trypsin-EDTA (0.05%), phenol red 
 9    Gibco(51800-035)                RPMI 1640 Medium, powder 
 10  Gibco (72400-021)               RPMI 1640 Medium (1X) liquid [with GlutaMAX™ I, 25 mM HEPES] 
 11 Gibco(22390-025)                 Ham's F-10 Nutrient Mix, HEPES 
  12  Gibco (35050-038)                GlutaMAX™ Supplement
  13 Gibco(25030024)                L-Glutamine (200 mM) 
  14 Gibco(22390-017)                 Ham's F-10 Nutrient Mix, HEPES 
15  Gibco(15140-122)                 Penicillin-Streptomycin (10,000 U/mL)
16  Gibco(18912-014)                 PBS Tablets
17 Gibco (10582-013)                Gurr Buffer Tablets
18   Gibco(15290-018)                 Fungizone® Antimycotic 
19 Thermo Scientific(156758)      flat bottom polystyrene (PS) cell culture tube( leyton tube)